enzyme sphihf (New England Biolabs)
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New England Biolabs
enzyme sphihf
Enzyme Sphihf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sphi+enzymes/SphI-HF/pm42097145-754-21-23
Average 96 stars, based on 183 article reviews
Enzyme Sphihf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sphi+enzymes/SphI-HF/pm42097145-754-21-23
Average 96 stars, based on 183 article reviews
enzyme sphihf - by Bioz Stars,
2026-09
96/100 stars
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Amplification:Article Title: Production of xylitol from glucose by a recombinant strain Article Snippet: The PCR was accomplished with an initial denaturation step of 30 sec at 98° C. followed by 30 cycles with 10 sec at 98° C./10 sec at 57° C./20 sec at 72° C., and a final extension step of 5 minutes at 72° C. The PCR product was separated on a 1% agarose gel, extracted and purified using the ZymocleanTM Gel DNA Recovery Kit (Zymo Research Corporation, Irvine, Calif.). .. The amplified LEU2 open reading frame was subsequently restriction digested with AscI and Article Title: Variable fitness effects of bacteriophage resistance mutations in Escherichia coli: implications for phage therapy. Article Snippet: .. To complement K15 capsule-related phage-resistant clones (ECP_3022, L17; ECP_3031, T10), the corresponding open reading frames (ORFs) were amplified from the WT strain 536 and cloned into the pUC18 plasmid (ampicillin 100 μg/mL) using BamHI and Article Title: Variable fitness effects of bacteriophage resistance mutations in Escherichia coli: implications for phage therapy Article Snippet: .. To complement K15 capsule-related phage-resistant clones ( ECP_3022 , L17; ECP_3031 , T10), the corresponding open reading frames (ORFs) were amplified from the WT strain 536 and cloned into the pUC18 plasmid (ampicillin 100 μg/mL) using BamHI and Ligation:Article Title: Dispersal ability predicts spatial genetic structure in native mammals persisting across an urbanization gradient Article Snippet: .. In brief, DNA was restriction‐digested with the MluCI and Clone Assay:Article Title: Variable fitness effects of bacteriophage resistance mutations in Escherichia coli: implications for phage therapy. Article Snippet: .. To complement K15 capsule-related phage-resistant clones (ECP_3022, L17; ECP_3031, T10), the corresponding open reading frames (ORFs) were amplified from the WT strain 536 and cloned into the pUC18 plasmid (ampicillin 100 μg/mL) using BamHI and Article Title: Variable fitness effects of bacteriophage resistance mutations in Escherichia coli: implications for phage therapy Article Snippet: .. To complement K15 capsule-related phage-resistant clones ( ECP_3022 , L17; ECP_3031 , T10), the corresponding open reading frames (ORFs) were amplified from the WT strain 536 and cloned into the pUC18 plasmid (ampicillin 100 μg/mL) using BamHI and Plasmid Preparation:Article Title: Variable fitness effects of bacteriophage resistance mutations in Escherichia coli: implications for phage therapy. Article Snippet: .. To complement K15 capsule-related phage-resistant clones (ECP_3022, L17; ECP_3031, T10), the corresponding open reading frames (ORFs) were amplified from the WT strain 536 and cloned into the pUC18 plasmid (ampicillin 100 μg/mL) using BamHI and Article Title: Variable fitness effects of bacteriophage resistance mutations in Escherichia coli: implications for phage therapy Article Snippet: .. To complement K15 capsule-related phage-resistant clones ( ECP_3022 , L17; ECP_3031 , T10), the corresponding open reading frames (ORFs) were amplified from the WT strain 536 and cloned into the pUC18 plasmid (ampicillin 100 μg/mL) using BamHI and Article Title: Production of xylitol from glucose by a recombinant strain Article Snippet: The amplification was accomplished with an initial denaturation step of 30 sec at 98° C. followed by 30 cycles with 10 sec at 98° C./10 sec at 61° C./15 sec at 72° C., and a final extension step of 5 minutes at 72° C. The PCR product was separated on a 1% agarose gel, extracted and purified using the ZymocleanTM Gel DNA Recovery Kit (Zymo Research Corporation, Irvine, Calif.). .. The 567 bp fragment was restriction digested with PstI and Article Title: Mfd protects against oxidative stress in Bacillus subtilis independently of its canonical function in DNA repair. Article Snippet: The PCR products were then resolved on 1% Agarose gel and fragments corresponding to 1.2 kb size were excised out of the gel and cleaned up using the Qiagen MinElute Gel Extraction Kit (Venlo, Netherlands). .. The cleanup product was then digested using SalI and Transformation Assay:Article Title: Mfd protects against oxidative stress in Bacillus subtilis independently of its canonical function in DNA repair. Article Snippet: The PCR products were then resolved on 1% Agarose gel and fragments corresponding to 1.2 kb size were excised out of the gel and cleaned up using the Qiagen MinElute Gel Extraction Kit (Venlo, Netherlands). .. The cleanup product was then digested using SalI and Subcloning:Article Title: Production of xylitol from glucose by a recombinant strain Article Snippet: The synthesized DNA fragment encoding the NADPH-specific xylitol dehydrogenase from Gluconobacter oxydans was delivered as 5 μg lyophilized plasmid DNA in a pMA-T derived vector (13AAYSYP, FIG. 3). .. For further subcloning, the gene was released by restriction cutting with AscI and |